João Loureiro


	Education: Graduation (1997-2002), Biology, Department of Biology, University of Aveiro, Portugal PhD (2002 – 2007): Biology, Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Portugal (Supervisor: Prof. Conceição Santos) and Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic (co-supervisor: Prof. Jaroslav Dolezel) PhD Thesis: "Flow cytometric approaches to study plant cytomics and genomics" Research Interests: Flow Cytometry: - Genome size estimations in plant species; - Ploidy stability of in vitro cultures; - Methodological issues (analyses of the effect of cytosolic compounds, nuclear isolation buffer's comparison, best practices, etc.) Plant functional cytomics Molecular cytogenetics; Biosystematics. Book Chapters : - Santos C, Loureiro, Lopes T, Pinto G (2007). Genetic fidelity analyses of in vitro propagated cork oak (Quercus suber L.). In: Mohan Jain S, Häggman H (eds.) Protocols for micropropagation of woody and fruit trees. Springer, Dordrecht, The Netherlands (in press - to be published on August 2007); - Loureiro J, Suda J, Doležel J, Santos C (2007). FLOWer – Plant DNA flow cytometry database. In: Doležel J, Greilhuber J, Suda J (eds.) Flow cytometry with plant cells. WILEY-VCH Verlag, Weinheim, Germany pp. 423-438; - Greilhuber J, Temsch E, Loureiro J (2007). Nuclear DNA content measurement. In: Doležel J, Greilhuber J, Suda J (eds.) Flow cytometry with plant cells. WILEY-VCH Verlag, Weinheim, Germany, pp. 67-101. Papers in International Journals: - Loureiro J, Rodriguez E, Doležel J, Santos C (2007). Two new nuclear isolation buffers for plant DNA flow cytometry – examination with 37 species. (Annals of Botany, in press) - Brito G, Loureiro J, Lopes T, Rodriguez E, Santos C (2007). Genetic characterisation of olive trees from Madeira Archipelago using flow cytometry and microsatellite markers. (Genetic Resources and Crop Evolution, in press) - Loureiro J, Kopecký D, Castro S, Santos C, Silveira P (2007). Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp. (Plant Systematics and Evolution, online, DOI: 10.1007/s00606-007-0564-8) - Loureiro J, Rodriguez E, Gomes A, Santos C (2007). Genome size estimations on Ulmus minor Mill., Ulmus glabra Huds. and Celtis australis L. using flow cytometry. Plant Biology, 9:541-544. - Castro S, Loureiro J, Rodriguez E, Silveira P, Navarro L, Santos C (2007). Evaluation of polysomaty and estimation of genome size in Polygala vayredae and P. calcarea using flow cytometry. Plant Science, 172:1131-1137. - Castro S, Loureiro J, Santos C, Ater M, Ayensa G, Navarro L (2007). Distribution of flower morphs, ploidy level and sexual reproduction of invasive weed Oxalis pes-caprae in the western area of the Mediterranean Region. Annals of Botany 99:507-517 - Loureiro J, Rodriguez E, Costa A, Santos C (2007). Nuclear DNA content estimations in wild olive (Olea europaea L. ssp. europaea var. sylvestris) and Portuguese cultivars of O. europaea using flow cytometry. Genetic Resources and Crop Evolution 54:21-25 - Loureiro J, Capelo A, Brito G, Rodriguez E, Silva S, Pinto G, Santos C (2007). Micropropagation of Juniperus phoenicea L. adult plants and ploidy stability analyses in micropropagated plants using flow cytometry. Biologia Plantarum 51(1):7-14 - Oliveira H, Loureiro J, Filipe L, Santos C, Ramalho-Santos J, Sousa M, Pereira ML (2006). Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis. Reproductive Toxicology 22:529-535 - Kopecký D, Loureiro J, Zwierzykowski Z, Ghesquière M, Doležel J (2006). Genome constitution and evolution in Lolium x Festuca hybrid cultivars (Festulolium). Theoretical and Applied Genetics 113:731-742 - Loureiro J, Rodriguez E, Doležel J, Santos C (2006). Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content. Annals of Botany 98:515-527 - Loureiro J, Rodriguez E, Doležel J, Santos C (2006). Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Annals of Botany 98:679-689 - Leal F, Loureiro J, Rodriguez E, Pais MS, Santos C, Pinto-Carnide O (2006). Nuclear DNA content of Vitis vinífera cultivars and ploidy level analyses of somatic embryo derived plants obtained after anther culture. Plant Cell Reports 25:978-985 - Lopes T, Pinto G, Loureiro J, Costa A, Santos C (2006). Determination of genetic stability in cork oak long term somatic embryogenetic cultures and derived plantlets using microsatellite markers. Tree Physiology 26:1145-1152 - Loureiro J, Pinto G, Lopes T, Doležel J, Santos C (2005). Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry. Planta 221:815-822 - Pinto G, Loureiro J, Lopes T, Santos C (2004). Analysis of genetic stability of Eucalyptus globulus Labill. somatic embryos by flow cytometry. Theoretical and Applied Genetics 109(4):580-587 - Conde P, Loureiro J, Santos C (2004). Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill. Plant Cell Reports 22:632-639 - Santos C, Falcão I, Pinto G, Oliveira H, Loureiro J (2002). Nutrient responses and glutamate and proline metabolism in sunflower plants and calli under Na₂SO₄ stress. Journal of Plant Nutrition and Soil Science 165:366-372 Papers in National Journals: - Loureiro J, Santos C (2004). Aplicação da citometria de fluxo ao estudo do genoma vegetal. Boletim de Biotecnologia 77:18-29 - Pinto G, Lopes T, Loureiro J, Valentim H, Costa A, Santos C (2004). Micropropagação de sobreiro: a importância dos métodos não convencionais. Biologia Vegetal e Agro-Industrial 1:49-67 Organization of scientific meetings: - Organization of the Workshop - Introdução à citometria de fluxo vegetal that was part of the XI Encontro Nacional de Estudantes de Biologia and took place in the Department of Biology, University of Aveiro in the 1st of April 2007 - Organization of the Practical Course - Flow cytometric and microscopic analysis of male reproductive cells that took place in the Department of Biology, University of Aveiro from 20th to 21st May 2005 - Organization of the Advanced Course - Flow cytometry applied to plant structural cytomics that took place in the Department of Biology, University of Aveiro from 20th to 21st May 2005 - Participation in the organization of the XXXIX Reunion of the Portuguese Society of Electron Microscopy and Cell Biology that took place in University of Aveiro from 4th to 6th of November 2004 - Organization of the Seminar - Applications of flow cytometry in Biology, Health and Environment that took place in University of Aveiro in 5th of May 2004 - Organization of the Seminar - GMO: what present, what future? that took place in University of Aveiro in 14th of April 2004 - Organization of Jornadas do Mar '99 that took place in University of Aveiro from 1st to 4th of October 1999

Education:

Graduation (1997-2002), Biology, Department of Biology, University of Aveiro, Portugal

PhD (2002 – 2007): Biology, Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Portugal (Supervisor: Prof. Conceição Santos) and Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic (co-supervisor: Prof. Jaroslav Dolezel)

PhD Thesis: "Flow cytometric approaches to study plant cytomics and genomics"

Research Interests:

Flow Cytometry:

- Genome size estimations in plant species;

- Ploidy stability of in vitro cultures;

- Methodological issues (analyses of the effect of cytosolic compounds, nuclear isolation buffer's comparison, best practices, etc.)

Plant functional cytomics
Molecular cytogenetics;
Biosystematics.

Book Chapters :

- Santos C, Loureiro, Lopes T, Pinto G (2007). Genetic fidelity analyses of in vitro propagated cork oak (Quercus suber L.). In: Mohan Jain S, Häggman H (eds.) Protocols for micropropagation of woody and fruit trees. Springer, Dordrecht, The Netherlands (in press - to be published on August 2007);

- Loureiro J, Suda J, Doležel J, Santos C (2007). FLOWer – Plant DNA flow cytometry database. In: Doležel J, Greilhuber J, Suda J (eds.) Flow cytometry with plant cells. WILEY-VCH Verlag, Weinheim, Germany pp. 423-438;

- Greilhuber J, Temsch E, Loureiro J (2007). Nuclear DNA content measurement. In: Doležel J, Greilhuber J, Suda J (eds.) Flow cytometry with plant cells. WILEY-VCH Verlag, Weinheim, Germany, pp. 67-101.

Papers in International Journals:

- Loureiro J, Rodriguez E, Doležel J, Santos C (2007). Two new nuclear isolation buffers for plant DNA flow cytometry – examination with 37 species. (Annals of Botany, in press)

- Brito G, Loureiro J, Lopes T, Rodriguez E, Santos C (2007). Genetic characterisation of olive trees from Madeira Archipelago using flow cytometry and microsatellite markers. (Genetic Resources and Crop Evolution, in press)

- Loureiro J, Kopecký D, Castro S, Santos C, Silveira P (2007). Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp. (Plant Systematics and Evolution, online, DOI: 10.1007/s00606-007-0564-8)

- Loureiro J, Rodriguez E, Gomes A, Santos C (2007). Genome size estimations on Ulmus minor Mill., Ulmus glabra Huds. and Celtis australis L. using flow cytometry. Plant Biology, 9:541-544.

- Castro S, Loureiro J, Rodriguez E, Silveira P, Navarro L, Santos C (2007). Evaluation of polysomaty and estimation of genome size in Polygala vayredae and P. calcarea using flow cytometry. Plant Science, 172:1131-1137.

- Castro S, Loureiro J, Santos C, Ater M, Ayensa G, Navarro L (2007). Distribution of flower morphs, ploidy level and sexual reproduction of invasive weed Oxalis pes-caprae in the western area of the Mediterranean Region. Annals of Botany 99:507-517

- Loureiro J, Rodriguez E, Costa A, Santos C (2007). Nuclear DNA content estimations in wild olive (Olea europaea L. ssp. europaea var. sylvestris) and Portuguese cultivars of O. europaea using flow cytometry. Genetic Resources and Crop Evolution 54:21-25

- Loureiro J, Capelo A, Brito G, Rodriguez E, Silva S, Pinto G, Santos C (2007). Micropropagation of Juniperus phoenicea L. adult plants and ploidy stability analyses in micropropagated plants using flow cytometry. Biologia Plantarum 51(1):7-14

- Oliveira H, Loureiro J, Filipe L, Santos C, Ramalho-Santos J, Sousa M, Pereira ML (2006). Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis. Reproductive Toxicology 22:529-535

- Kopecký D, Loureiro J, Zwierzykowski Z, Ghesquière M, Doležel J (2006). Genome constitution and evolution in Lolium x Festuca hybrid cultivars (Festulolium). Theoretical and Applied Genetics 113:731-742

- Loureiro J, Rodriguez E, Doležel J, Santos C (2006). Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content. Annals of Botany 98:515-527

- Loureiro J, Rodriguez E, Doležel J, Santos C (2006). Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Annals of Botany 98:679-689

- Leal F, Loureiro J, Rodriguez E, Pais MS, Santos C, Pinto-Carnide O (2006). Nuclear DNA content of Vitis vinífera cultivars and ploidy level analyses of somatic embryo derived plants obtained after anther culture. Plant Cell Reports 25:978-985

- Lopes T, Pinto G, Loureiro J, Costa A, Santos C (2006). Determination of genetic stability in cork oak long term somatic embryogenetic cultures and derived plantlets using microsatellite markers. Tree Physiology 26:1145-1152

- Loureiro J, Pinto G, Lopes T, Doležel J, Santos C (2005). Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry. Planta 221:815-822

- Pinto G, Loureiro J, Lopes T, Santos C (2004). Analysis of genetic stability of Eucalyptus globulus Labill. somatic embryos by flow cytometry. Theoretical and Applied Genetics 109(4):580-587

- Conde P, Loureiro J, Santos C (2004). Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill. Plant Cell Reports 22:632-639

- Santos C, Falcão I, Pinto G, Oliveira H, Loureiro J (2002). Nutrient responses and glutamate and proline metabolism in sunflower plants and calli under Na₂SO₄ stress. Journal of Plant Nutrition and Soil Science 165:366-372

Papers in National Journals:

- Loureiro J, Santos C (2004). Aplicação da citometria de fluxo ao estudo do genoma vegetal. Boletim de Biotecnologia 77:18-29

- Pinto G, Lopes T, Loureiro J, Valentim H, Costa A, Santos C (2004). Micropropagação de sobreiro: a importância dos métodos não convencionais. Biologia Vegetal e Agro-Industrial 1:49-67

Organization of scientific meetings:

- Organization of the Workshop - Introdução à citometria de fluxo vegetal that was part of the XI Encontro Nacional de Estudantes de Biologia and took place in the Department of Biology, University of Aveiro in the 1st of April 2007

- Organization of the Practical Course - Flow cytometric and microscopic analysis of male reproductive cells that took place in the Department of Biology, University of Aveiro from 20th to 21st May 2005

- Organization of the Advanced Course - Flow cytometry applied to plant structural cytomics that took place in the Department of Biology, University of Aveiro from 20th to 21st May 2005

- Participation in the organization of the XXXIX Reunion of the Portuguese Society of Electron Microscopy and Cell Biology that took place in University of Aveiro from 4th to 6th of November 2004

- Organization of the Seminar - Applications of flow cytometry in Biology, Health and Environment that took place in University of Aveiro in 5th of May 2004

- Organization of the Seminar - GMO: what present, what future? that took place in University of Aveiro in 14th of April 2004

- Organization of Jornadas do Mar '99 that took place in University of Aveiro from 1st to 4th of October 1999